Controlling pH in embryo culture media is often perceived as difficult by embryologists. Still, to maintain the optimal physiological pH range of 7.20 – 7.40 is essential to maintain oocyte and embryo viability. Many functions in embryo cells such as glycolysis, protein metabolism, cytoskeletal dynamics, cell transport and epigenetic functions are all dependent on pH.
This is an abbreviation of Jason Swain (2019) publication with emphasis on the oil used for medium overlay written by Ann-Helene Jakobsson, Vitrolife.
Perfect oil for IVF should do nothing but protect the culture. It should not have any negative influence on the gametes, embryos or media by adding unwanted or removing necessary components. However not all oils are or act the same. In this blog post I will guide you through different types of oil and things to consider when choosing oil for your IVF culture.
This blog focuses on EmbryoGlue® as there are now three Cochrane reviews showing improved clinical outcome when using this transfer medium, which contains a functional concentration of hyaluronan. The Number Needed to Treat (number of patients treated with EmbryoGlue to achieve an additional live birth) is reported to be around 17 patients, so perhaps the use of this specifically created transfer medium should not be considered an add-on, but rather a standard of care.
This year, online was the place to be when participating in the 76th annual meeting of ASRM. In this blog post, thoughts and reflections from the Vitrolife team have been collected on some of the scientific content presented at the meeting.
What has lactate (aka lactic acid) got to do with embryos? Most people are familiar with this carboxylic acid only in the context of it as the end product of glucose use through glycolysis that builds up in your muscles when you exercise at the gym! Well, it transpires that lactate is actually a key regulator of embryo function, with new roles emerging as our understanding of cell physiology increases. In other words, it is far more important than we originally thought.
The 36th annual meeting of ESHRE took place online this year, with more than 12,000 registered participants. Like last year, the Vitrolife team has put together our thoughts and reflections on some of the scientific content from the meeting. We also offer the opportunity to watch our recorded scientific symposium again.
What is artificial intelligence?
Artificial intelligence is widely used and affects our daily lives even if we do not notice. From smart phone tools and personalised shopping experiences to assisted airline operations and medical diagnosis. When the appropriate method is chosen and trained on data that sufficiently represents the intended use, artificial intelligence makes our life easier and more comfortable.
Chromosome aneuploidy (abnormal chromosome copy number) is a major cause of IVF failure, pregnancy loss and in rare cases, affected live births. Preimplantation genetic testing for aneuploidy (PGT-A), following culture to the blastocyst stage, trophectoderm biopsy, vitrification and testing by next generation sequencing (NGS) based methods for chromosome copy number analysis, is now widely used to identify embryos with normal chromosome copy number (euploid).
The beginning of a year is always a time for reflection on the achievements of the past year and an opportunity to look forward to what the coming year may bring. And for the newly formed Genomics Division here at Vitrolife, 2019 was certainly a busy time that has flown by. The team was well represented at all of the main reproductive genetics and IVF annual meetings including Preimplantation Genetic Diagnosis International Society (PGDIS) meeting in Geneva and Controversies in Preconception, Preimplantation and Prenatal Genetics (CoGen) in Paris. At ESHRE in Vienna, Genomics was represented on the Vitrolife stand, which was a great opportunity to meet customers face-to-face, but equally important for us, to get to know all of our colleagues in other areas! There was a well-attended lunch time symposium at which I represented genomics and, in the evening, I had a lot of fun giving the after-dinner talk reminiscing on 30 plus years of my involvement in the field, since we published the first clinical pregnancies following preimplantation genetic diagnosis or PGD, as it was known then, for a range of X-linked inherited diseases, including Duchenne muscular dystrophy.